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| Name | Position | Size (bp) | Type | Description | Application notes | View |
|---|---|---|---|---|---|---|
| 5' ITR | 1-103 | 103 |
|
Adenovirus 5' inverted terminal repeat
|
Allows replication of the viral genome and its packaging into virus.
|
|
| misc_feature1 | 104-190 | 87 |
|
|
|
|
| Ψ | 191-370 | 180 |
|
HIV-1 packaging signal
|
Allows packaging of viral RNA into virus.
|
|
| misc_feature | 371-510 | 140 |
|
|
|
|
| attB4 | 511-531 | 21 |
|
|
|
|
| CBh | 532-1329 | 798 |
|
CMV early enhancer fused to modified chicken β-actin promoter
|
Strong promoter.
|
|
| attB1 | 1330-1353 | 24 |
|
|
|
|
| Kozak | 1354-1359 | 6 |
|
Kozak translation initiation sequence
|
Facilitates translation initiation of ATG start codon downstream of the Kozak sequence.
|
|
| EGFP | 1360-2079 | 720 |
|
Enhanced green fluorescent protein; codon optimized based on a variant of wild type GFP from the jellyfish Aequorea victoria
|
Commonly used green fluorescent protein; ranked high in brightness, photostability and pH stability among all fluorescent proteins.
|
|
| attB2 | 2080-2103 | 24 |
|
|
|
|
| BGH pA | 2104-2328 | 225 |
|
Bovine growth hormone polyadenylation signal
|
Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase.
|
|
| hPGK promoter | 2329-2833 | 505 |
|
Human phosphoglycerate kinase 1 promoter
|
Medium-strength promoter.
|
|
| Kozak | 2834-2839 | 6 |
|
Kozak translation initiation sequence
|
Facilitates translation initiation of ATG start codon downstream of the Kozak sequence.
|
|
| EGFP | 2840-3559 | 720 |
|
Enhanced green fluorescent protein; codon optimized based on a variant of wild type GFP from the jellyfish Aequorea victoria
|
Commonly used green fluorescent protein; ranked high in brightness, photostability and pH stability among all fluorescent proteins.
|
|
| attB3 | 3560-3580 | 21 |
|
|
|
|
| misc_feature5 | 3581-3598 | 18 |
|
|
|
|
| TK pA | 3599-3870 | 272 |
|
Herpes Simplex Virus thymidine kinase polyadenylation signal
|
Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase.
|
|
| misc_feature | 3871-3887 | 17 |
|
|
|
|
| ΔAd5 | 3888-33881 | 29994 |
|
Deleted adenovirus serotype 5 genome sequence
|
Portion of adenovirus serotype 5 genome between the two ITRs minus the E1A, E1B and E3 regions.
|
|
| misc_feature | 33882-34327 | 446 |
|
|
|
|
| 3' ITR | 34328-34430 | 103 |
|
Adenovirus 3' inverted terminal repeat
|
Allows replication of the viral genome and its packaging into virus.
|
|
| PacI | 34431-34438 | 8 |
|
PacI restriction site
|
Allows linearization of vector.
|
|
| misc_feature | 34439-34653 | 215 |
|
|
|
|
| pBR322 ori |
complement (34654-35242) | 589 |
|
pBR322 origin of replication
|
Facilitates plasmid replication in E. coli; regulates low-copy plasmid number when Rop protein is presence (15-20) and median-copy plasmid number when Rop is absent (100-300).
|
|
| misc_feature8 | 35243-35393 | 151 |
|
|
|
|
| Ampicillin |
complement (35394-36254) | 861 |
|
Ampicillin resistance gene
|
Allows E. coli to be resistant to ampicillin.
|
|
| misc_feature | 36255-36509 | 255 |
|
|
|
|
| PacI | 36510-36517 | 8 |
|
PacI restriction site
|
Allows linearization of vector.
|
|
Note: Components added by user are listed in bold red text.